Room temperature is a general term describing common indoor temperatures. It is usually in the range of 20 °C (68 °F or 293 K) to 25 °C (77 °F or 298 K).
The American Society of Heating, Refrigerating and Air-Conditioning Engineers (ASHRAE) has listings for suggested temperatures and air flow rates in different types of buildings and different environmental circumstances. A comfortable room temperature depends on individual needs and other factors.
Room temperature implies a temperature inside a temperature-controlled building. Ambient temperature simply means "the temperature of the surroundings" and will be the same as room temperature indoors. In many languages, such as Spanish, there is an expression for ambient temperature, but no distinct translation for room temperature.